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Test Code TLPF T-Cell Lymphoma, FISH, Varies


Advisory Information


This assay detects chromosome abnormalities observed in the blood or bone marrow of patients with T-cell lymphoma.

-For testing paraffin-embedded tissue samples from patients with T-cell lymphoma, see TLYM / T-Cell Lymphoma, FISH, Tissue.

-For patients with T-cell acute lymphoblastic leukemia/lymphoma, order TALLF / T-Cell Acute Lymphoblastic Leukemia [T-ALL], FISH, Varies.



Shipping Instructions


Advise Express Mail or equivalent if not on courier service.



Necessary Information


1. Provide a reason for referral with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.

2. A pathology or flow cytometry report may be requested by the Genomics Laboratory to optimize testing and aid in interpretation of results.



Specimen Required


Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Bone marrow

Container/Tube: Green top (sodium heparin)

Specimen Volume: 1-2 mL

Collection Instructions: Invert several times to mix bone marrow.

 

Acceptable:

Specimen Type: Blood

Container/Tube: Green top (sodium heparin)

Specimen Volume: 7-10 mL

Collection Instructions: Invert several times to mix blood.


Forms

If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.

Useful For

Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with various T-cell lymphomas

 

Tracking known chromosome abnormalities and response to therapy in patients with T-cell lymphoma

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
_I099 Interphases, 25-99 No, (Bill Only) No
_I300 Interphases, >=100 No, (Bill Only) No
_IL25 Interphases, <25 No, (Bill Only) No
_PADD Probe, +1 No, (Bill Only) No
_PB02 Probe, +2 No, (Bill Only) No
_PB03 Probe, +3 No, (Bill Only) No
_PBCT Probe, +2 No, (Bill Only) No

Testing Algorithm

This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.

 

When this test and flow cytometry testing for leukemia/lymphoma are ordered concurrently, the flow cytometry result will be utilized to determine if sufficient clonal T-cells are available for FISH testing. If the result does not identify a sufficient clonal T-cell population, this FISH test order will be canceled and no charges will be incurred.

 

If FISH testing proceeds, probes will be performed based on the lymphoma subtype suspected/identified utilizing the table located in Clinical Information.

 

If the patient is being tracked for known abnormalities, indicate which probes should be used.

 

The following probe sets are available within the T-cell lymphoma FISH profile:

14q32.1 rearrangement, TCL1A

/i(7q)/ -7/7q-, D7S486/D7Z1

+8, D8Z2/MYC

 

If T-cell prolymphocytic leukemia/lymphoma (T-PLL) is indicated, TCL1A rearrangement is performed, with reflex to the TRAD FISH probe if TCL1A is negative,

 

See Bone Marrow Staging for Known or Suspected Malignant Lymphoma Algorithm in Special Instructions.

Method Name

Fluorescence In Situ Hybridization (FISH)

Reporting Name

T-cell Lymphoma, FISH, B/BM

Specimen Type

Varies

Specimen Minimum Volume

Blood: 2 mL
Bone Marrow: 1 mL

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Refrigerated 

Reject Due To

No specimen should be rejected.

Reference Values

An interpretive report will be provided.

Day(s) and Time(s) Performed

Samples processed Monday through Sunday. Results reported Monday through Friday, 8 a.m.-5 p.m. CT.

Analytic Time

7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

88271x2, 88291-DNA probe, each (first probe set), Interpretation and report

88271x2-DNA probe, each; each additional probe set (if appropriate)

88271x1-DNA probe, each; coverage for sets containing 3 probes (if appropriate)

88271x2-DNA probe, each; coverage for sets containing 4 probes (if appropriate)

88271x3-DNA probe, each; coverage for sets containing 5 probes (if appropriate)

88274 w/modifier 52-Interphase in situ hybridization, <25 cells, each probe set (if appropriate)

88274-Interphase in situ hybridization, 25 to 99 cells, each probe set (if appropriate)

88275-Interphase in situ hybridization, 100 to 300 cells, each probe set (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TLPF T-cell Lymphoma, FISH, B/BM In Process

 

Result ID Test Result Name Result LOINC Value
52009 Result Summary 50397-9
52011 Interpretation 69965-2
52010 Result Table 36908-2
54566 Result 62356-1
CG719 Reason for Referral 42349-1
CG720 Specimen 31208-2
52012 Source 31208-2
52013 Method 49549-9
55118 Additional Information 48767-8
53849 Disclaimer 62364-5
52014 Released By 18771-6