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Test Code TCRB T-Cell Receptor V-Beta Repertoire Analysis, Spectratyping, Blood


Ordering Guidance


Mayo Clinic Laboratory Director/Consultant approval is required prior to ordering this test in patients greater than 40 years of age.



Additional Testing Requirements


Additional tests that could be ordered in conjunction with this test include:

-TRECS / T-Cell Receptor Excision Circles (TREC) Analysis, Blood

-CD4RT / CD4 T-Cell Recent Thymic Emigrants, Blood

-TCP / T-Cell Subsets, Naive, Memory, and Activated, Blood



Shipping Instructions


Specimens must be received in the laboratory on weekdays and by 4 p.m. on Friday. Collect and package specimen as close to shipping time as possible.

 

It is recommended that specimens arrive within 24 hours of collection.

 

Samples arriving over the weekend and/or on observed holidays may be canceled.



Necessary Information


Ordering physician's name and phone number are required.

 

TCR V beta Spectratyping Assay Patient Information (T719) is required. Testing will proceed without the form; however, results will be held under the information is received.



Specimen Required


For serial monitoring, it is recommended to perform specimen collection at the same time of day, if possible.

 

Supplies: Ambient Shipping Box-Critical Specimens Only (T668)

Specimen Type: Blood

Container/Tube: Lavender top (EDTA)

Specimen Volume:

Adults: 10 mL

Pediatrics:

-Preferred volume for >1 year: 3 mL

-Preferred volume for ≤1 year: 1 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.


Useful For

Assessment of T-cell receptor diversity in various clinical contexts including inborn errors of immunity (formerly primary immunodeficiencies), monitoring immune reconstitution post-hematopoietic stem cell transplantation, and temporal assessment of repertoire changes in autoimmune diseases and viral infections

Method Name

Molecular TCR Vb-CDR3 Fragment Length Analysis

Reporting Name

TCRVB Spectratyping, B

Specimen Type

Whole Blood EDTA

Specimen Minimum Volume

Adults: 5 mL
Pediatrics: 1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Ambient 48 hours PURPLE OR PINK TOP/EDTA

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Clotted Reject

Reference Values

References values will be provided in the patient report.

Day(s) Performed

Varies

Report Available

20 to 24 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

81340-TRG (T cell antigen receptor, beta) (eg, leukemia and lymphoma), gene rearrangement analysis to detect abnormal clonal population(s); using amplification methodology (eg, polymerase chain reaction)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TCRB TCRVB Spectratyping, B 21751-3

 

Result ID Test Result Name Result LOINC Value
616418 CD3 T Cells 8122-4
616419 CD4 T Cells 24467-3
616420 CD8 T Cells 14135-8
615831 Family 2 Diversity Ratio 82501-8
615832 Family 3-1 Diversity Ratio 82500-0
615833 Family 4 Diversity Ratio 82499-5
615834 Family 5 Diversity Ratio 82498-7
615835 Family 6 Diversity Ratio 82497-9
615836 Family 7 Diversity Ratio 82496-1
615837 Family 9 Diversity Ratio 82495-3
615838 Family 10 Diversity Ratio 82494-6
615839 Family 11 Diversity Ratio 82493-8
615840 Family 12 Diversity Ratio 82492-0
615841 Family 13 Diversity Ratio 82491-2
615842 Family 14 Diversity Ratio 82490-4
615843 Family 15 Diversity Ratio 82489-6
615844 Family 16 Diversity Ratio 82488-8
615845 Family 18 Diversity Ratio 82487-0
615846 Family 19 Diversity Ratio 82486-2
615847 Family 20-1 Diversity Ratio 82485-4
615848 Family 24-1 Diversity Ratio 82484-7
615849 Family 25 Diversity Ratio 82483-9
615850 Family 27 Diversity Ratio 82482-1
615851 Family 28 Diversity Ratio 82481-3
615852 Family 29 Diversity Ratio 82480-5
615853 Family 30 Diversity Ratio 82479-7
615854 Interpretation 69047-9
615855 Additional Information 48767-8
615856 Method 85069-3
615857 Disclaimer 62364-5
615858 Released By 18771-6