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Test Code MYD88 MYD88, L265P, Somatic Gene Mutation, DNA Allele-Specific PCR, Varies


Shipping Instructions


Whole blood or bone marrow specimens must arrive within 10 days of collection.



Necessary Information


 



Specimen Required


Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Bone marrow aspirate

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD), green top (sodium heparin)

Specimen Volume: 2 mL

Collection Instructions:

1. Invert several times to mix bone marrow.

2. Send bone marrow specimen in original tube. Do not aliquot.

3. Label specimen as bone marrow.

Specimen Stability Information: Ambient (preferred) 10 days/Refrigerated 10 days

 

Specimen Type: Whole blood

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD), green top (sodium heparin)

Specimen Volume: 3 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send whole blood specimen in original tube. Do not aliquot.

3. Label specimen as blood.

Specimen Stability Information: Ambient (preferred) 10 days/Refrigerated 10 days

 

Specimen Type: Paraffin-embedded tissue

Container/ Tube: Paraffin block

Collection Instructions:

1. Decalcified specimens (eg, bone marrow core biopsies) are not acceptable.

2. Indicate specimen source.

Specimen Stability Information: Ambient

Additional Information: If the quality of the biopsy specimen is poor, testing should not be ordered. Testing may be canceled if DNA requirements are inadequate.

 

Acceptable:

Specimen Type: Tissue slide

Slides: 20 unstained slides

Container/ Tube: Transport in plastic slide holders.

Collection Instructions:

1. Send 20 unstained, nonbaked slides with 5-micron thick sections of tissue.

2. Decalcified specimens (eg, bone marrow core biopsies) are not acceptable.

3. Indicate specimen source.

Specimen Stability Information: Ambient

Additional Information: Testing may be canceled if resultant extracted DNA does not meet concentration requirements.

 

Specimen Type: Frozen tissue

Container/Tube: Plastic container

Specimen Volume: 100 mg

Collection Instructions:

1. Freeze tissue within 1 hour of collection

2. Indicate specimen source.

Specimen Stability Information: Frozen

 

Specimen Type: Extracted DNA

Container/Tube: 1.5- to 2-mL tube

Specimen Volume: Entire specimen

Collection Instructions:

1. DNA must be extracted within 7 days of collection.

2. Label specimen as extracted DNA and source of specimen.

3. Provide volume and concentration of DNA on label.

Specimen Stability Information: Frozen (preferred)/Refrigerated/Ambient

Additional Information: DNA must be extracted in a CLIA-certified laboratory or equivalent and must be extracted from a specimen type listed as acceptable for this test (including applicable anticoagulants). We cannot guarantee that all extraction methods are compatible with this test. If testing fails, one repeat will be attempted, and if unsuccessful, the test will be reported as failed and a charge will be applied.


Forms

1. Hematopathology Patient Information (T676)

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.

Useful For

Establishing the diagnosis of lymphoplasmacytic lymphoma/Waldenstrom macroglobulinemia

 

Helping to distinguish lymphoplasmacytic lymphoma/Waldenstrom macroglobulinemia (low-grade B-cell lymphoma) from other subtypes

Method Name

Allele-Specific Polymerase Chain Reaction (PCR)

Reporting Name

MYD88 L265P Gene Mutation Analysis

Specimen Type

Varies

Specimen Minimum Volume

Whole blood, bone marrow aspirate, body fluid: 0.5 mL; Frozen tissue: 50 mg; Extracted DNA: 50 microliters (mcL) at 20 ng/mcL; Tissue slides: 10 unstained slides

Specimen Stability Information

Specimen Type Temperature Time
Varies Varies 10 days

Reject Due To

Gross hemolysis Reject
B5-fixed tissues Reject
Decalcified bone marrow core biopsies Reject
Frozen tissue Reject
Methanol acetic acid (MAA)-fixed pellets Reject
Moderately to severely clotted Reject
Paraffin shavings Reject

Reference Values

Variant present or absent based on expected alteration polymerase chain reaction product size. Concurrent amplification of wild type MYD88 fragment determined for sample amplification integrity. MYD88 gene (NCBI accession NM_002468.4)

Day(s) Performed

Monday through Friday

Report Available

5 to 8 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

81305

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MYD88 MYD88 L265P Gene Mutation Analysis 82140-5

 

Result ID Test Result Name Result LOINC Value
MP021 Specimen Type 31208-2
36308 Final Diagnosis 82140-5
621251 MYD88 Cancel 77202-0