Test Code LD_I Lactate Dehydrogenase (LDH) Isoenzymes, Serum
Necessary Information
Patient's age is required.
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 2 mL divided into 2 containers each containing 1 mL
Collection Instructions: Centrifuge and aliquot serum into 2 plastic vials, each containing 1 mL, within 2 hours of collection.
Forms
Useful For
Investigating a variety of diseases involving the heart, liver, muscle, kidney, lung, and blood
Differentiating heart-synthesized lactate dehydrogenase (LDH) from liver and other sources
Investigating unexplained causes of LDH elevations
Detection of macro-LDH
Profile Information
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
LD | Lactate Dehydrogenase (LD), S | Yes | Yes |
LDI | LD Isoenzymes, S | No | Yes |
Special Instructions
Method Name
LDI: Electrophoresis Densitometry
LD: Photometric Rate Reaction
Reporting Name
Lactate Dehydrogenase Isoenzymes, SSpecimen Type
SerumSpecimen Minimum Volume
0.75 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Ambient (preferred) | 7 days | |
Refrigerated | 48 hours |
Reject Due To
Gross hemolysis | Reject |
Gross lipemia | OK |
Reference Values
LACTATE DEHYDROGENASE (LD)
1-30 days: 135-750 U/L
31 days-11 months: 180-435 U/L
1-3 years: 160-370 U/L
4-6 years: 145-345 U/L
7-9 years: 143-290 U/L
10-12 years: 120-293 U/L
13-15 years: 110-283 U/L
16-17 years: 105-233 U/L
≥18 years: 122-222 U/L
LD ISOENZYMES
I (fast band): 17.5-28.3%
II: 30.4-36.4%
III: 19.2-24.8%
IV: 9.6-15.6%
V (slow band): 5.5-12.7%
Day(s) Performed
Monday, Wednesday, Friday
Report Available
3 to 6 daysPerforming Laboratory

Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
83615
83625
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
LD_I | Lactate Dehydrogenase Isoenzymes, S | 42929-0 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
LD | Lactate Dehydrogenase (LD), S | 14804-9 |
305 | LD Isoenzymes, S | 49279-3 |
3344 | Total LD Activity | 14804-9 |
2282 | I, Heart | 2536-1 |
2283 | II | 2539-5 |
2285 | III | 2542-9 |
2286 | IV | 2545-2 |
2287 | V, Liver | 2548-6 |