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Test Code HIVPCR HIV-1 RNA Quantification by PCR, Plasma

Methodology

Reverse Transcription-Polymerase Chain Reaction (RT-PCR)

Specimen Requirements

Draw blood in a lavender-top (EDTA) tube(s). Green-top (heparin) tube is not acceptable. Spin down, remove plasma from cells within 6 hours, and send 2.5 mL (minimum volume: 1.2 mL) of EDTA plasma refrigerated. If there is a delay in transport of ≥5 hours, send specimen frozen in plastic vial.

Note:  This test is intended to be used to monitor known HIV-positive infections. It is not intended for primary detection of HIV infections except in children <18 months of age when serologic tests are not useful.

Reference Values

HIV-1 RNA Not Detected

Result range of the assay is 20 to 10,000,000 copies/mL (1.30 to 7.00 log copies/mL).

Day(s) Test Set Up

Varies; 1 time per week

CPT Coding

87536

Secondary ID

HHA1515

Performing Laboratory

Huntsville Hospital Laboratory

LOINC Coding

Test Name LOINC Code

HIV-1 RNA Quantification by PCR

 5018-7