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Test Code HIGA Heavy-Light Chain IgA Kappa/IgA Lambda, Immunofluorescence

Ordering Guidance

If additional interpretation/analysis is needed, request PATHC / Pathology Consultation along with this test and send the corresponding renal pathology light microscopy and immunofluorescence (IF) slides (or IF images on a CD), electron microscopy images (prints or CD), and the pathology report.

Shipping Instructions

1. Advise shipping specimens in Styrofoam transportation coolers to avoid extreme hot or cold temperatures to ensure specimens are received at required specimen stability temperature.

2. Attach the green pathology address label included in the kit to the outside of the transport container.

Specimen Required

Preferred: Frozen tissue

Supplies: Renal Biopsy Kit (T231)

Specimen Type: Kidney tissue

Container/Tube: Renal Biopsy Kit, Zeus/Michel's

Specimen Volume: Entire specimen

Collection Instructions: Collect specimens according to the instructions in Renal Biopsy Procedure for Handling Tissue for Light Microscopy (LM), Immunofluorescent Histology (IF), and Electron Microscopy (EM) in Special Instructions.

Additional Information: If standard immunoglobulin and complement immunofluorescence has already been performed, submit the residual frozen tissue (must contain glomeruli) on dry ice.


Acceptable: Tissue slides

Collection Instructions: Submit 2 frozen tissue unstained positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick. Ship on dry ice.


Renal Biopsy Patient Information in Special Instructions

Useful For

Immunofluorescent staining of IgA kappa and IgA lambda to aid in the diagnosis of monoclonal gammopathy-associated nephropathies

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IFPCI IF Initial No No
IFPCA IF Additional No No

Testing Algorithm

For the initial immunofluorescence (IF) stain performed, the appropriate bill-only test ID will be added and charged (IFPCI). For each additional IF stain performed, an additional bill-only test ID will be added and charged (IFPCA).

Method Name

Direct Immunofluorescence

Reporting Name

HLC IgA Kappa Lambda IF

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time Special Container
Special Frozen (preferred)

Reject Due To

  All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Day(s) Performed

Monday through Friday

Report Available

1 to 2 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

88346-Primary IF

88350-If add'l IF

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HIGA HLC IgA Kappa Lambda IF In Process


Result ID Test Result Name Result LOINC Value
610380 Interpretation 50595-8
610381 Participated in the Interpretation No LOINC Needed
610382 Report electronically signed by 19139-5
610383 Addendum 35265-8
610384 Gross Description 22634-0
610385 Material Received 94736-6
610386 Disclaimer 62364-5
610387 Case Number 80398-1

Reference Values

An interpretive report will be provided.