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Test Code HCPCR Hepatitis C Virus (HCV) RNA Detection and Quantification by Real-Time Reverse Transcription-PCR (RT-PCR), Plasma or Serum

Methodology

Real-Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR)

(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

Specimen Requirements

Submit only 1 of the following specimens:

 

Plasma

Container/Tube: Lavender-top (EDTA) tube(s)-Heparinized specimen is not acceptable.

Specimen Volume: 2.2 mL (minimum volume: 1.2 mL) of plasma

Collection Instructions: Spin down within 6 hours of draw, immediately refrigerate specimen, and freeze specimen within 12 hours of draw. Send specimen in plastic vial.

Note: 1. This test is intended to detect acute and chronic HCV infection and to monitor response to anti-HCV therapy.

2. Label specimen appropriately (plasma).

 

Serum

Container/Tube: Serum gel tube(s)-Plain, red-top tube is not acceptable.

Specimen Volume: 2.2 mL (minimum volume: 1.2 mL) of serum

Collection Instructions: Spin down within 6 hours of draw, immediately refrigerate specimen, and freeze specimen within 12 hours of draw. Send specimen in plastic vial.

Note: 1. This test is intended to detect acute and chronic HCV infection and to monitor response to anti-HCV therapy.

2. Label specimen appropriately (serum).

Reference Values

HCV RNA not detected

Day(s) Test Set Up

Varies; 2 times per week

Secondary ID

HHA1506

Performing Laboratory

Huntsville Hospital Laboratory

Specimen Transport Temperature

Frozen

Test Classification and CPT Coding

87522