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Test Code F8 Factor VIII Assay

Performing Laboratory

Huntsville Hospital Laboratory

Methodology

Fibrin Strand Detection

Specimen Requirements

1. Draw blood in a blue-top (citrate) tube(s). Spin down, remove plasma, spin plasma again, and place 0.5 mL of citrate platelet-poor plasma in plastic vial. (Glass vial is not acceptable.)

Note:  Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.
2. Freeze specimen immediately at ≤-40° C, if possible.
3. Send specimen frozen.
Note:  Each coagulation assay requested should have its own vial.

Specimen Stability Information

 

Specimen Type

Temperature

Time

Platelet poor plasma, citrated

RT, spun

4 hours

 

Reject Due To

 

Hemolysis

Mild:

ok

Gross:

Reject

Lipemia

Mild:

ok

Gross:

ok

Icterus

Mild:

ok

Gross:

ok

 

Reference Values

50-150 %

Day(s) Test Set Up

Monday, Friday

CPT Coding

85240

Secondary ID

HHA1239

LOINC Coding

Test Name LOINC Code

Factor VIII Assay

3209-4