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Test Code EBF Electrophoresis, Protein, Body Fluid

Specimen Required

Container/Tube: Sterile container

Specimen Volume: 10 mL

Collection Instructions: Centrifuge to remove any cellular material.

Additional Information: Indicate specimen source; acceptable body fluid types are peritoneal (abdominal, ascites, paracentesis, peritoneal), pleural (chest, thoracentesis, pleural), drain (drainage, Jackson-Pratt (JP) drain), pericardial, spinal (CSF or ventricular), synovial (joint, knee, elbow), and vitreous fluids

Useful For

Monitoring patient's body fluid proteins


Aiding in the diagnosis of monoclonal gammopathies, when used in conjunction with immunofixation of the patient's serum


Detecting oligoclonal banding in spinal fluid (the preferred test for detecting oligoclonal bands in spinal fluid is OLIG / Oligoclonal Banding, Serum and Spinal Fluid)

Profile Information

Test ID Reporting Name Available Separately Always Performed
FLD4 Fluid Type No Yes
TP1 Protein, Total Yes, (TPBF) Yes
ELPBF Protein Electrophoresis, BF No Yes

Method Name

Agarose Gel Electrophoresis

Reporting Name

Electrophoresis, Protein, BF

Specimen Type

Body Fluid

Specimen Minimum Volume

2 mL

Specimen Stability Information

Specimen Type Temperature Time
Body Fluid Refrigerated (preferred) 7 days
  Frozen  14 days
  Ambient  72 hours

Reject Due To








Body fluid types other than those listed in Specimen Required

Reference Values

Not applicable

Day(s) and Time(s) Performed

Monday through Saturday; 12 p.m.

Analytic Time

1 day

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

See Individual Test IDs

CPT Code Information

84166, 84157

LOINC Code Information

Test ID Test Order Name Order LOINC Value
EBF Electrophoresis, Protein, BF 88698-6


Result ID Test Result Name Result LOINC Value
2802 Albumin 88709-1
TP1 Protein, Total 2881-1
FLD4 Fluid Type 14725-6
2803 Alpha 1-Globulin 17812-9
2804 Alpha 2-Globulin 17814-5
2805 Beta-Globulin 17816-0
2806 Gamma-Globulin 17818-6