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Test Code DIFF Differential Leukocyte Count, Blood

Methodology

Manual—Microscopic Examination of Cells
Includes neutrophilic segs, lymphocytes, monocytes, eosinophils, basophils, erythrocyte morphology, and platelets.

Specimen Requirements

Specimen must arrive within 24 hours of draw.

 

Draw blood in a lavender-top (EDTA) tube(s) or a CAPIJECT® (EDTA) tube(s), and send 5 mL of EDTA whole blood. Forward unprocessed whole blood promptly.

Specimen Stability Information

 

Specimen Type

Temperature

Time
Whole blood, EDTA RT 24 hrs

 

Refrigerated

48 hrs

 

Reference Values

 

Absolute Neutrophils (103/uL)
Age Male Female
>0d 7.80-14.50 7.80-14.50
>1d 1.75-7.20 1.75-7.20
>5d 1.80-5.40 1.80-5.40
>28d 2.10-8.00 2.10-8.00
≥1yr 1.50-6.90 1.50-6.90
≥4yr 1.50-7.60 1.50-7.60
≥10yr 1.80-7.20 1.80-7.20
≥18yr 1.50-7.90 1.50-7.90
Absolute Lymphocyte (103/uL)
Age Male Female
>0d 2.00-7.20 2.00-7.20
>1d 1.90-6.60 1.90-6.60
>5d 2.85-9.10 2.85-9.10
>28d 4.00-10.50 4.00-10.50
≥1yr 2.50-8.60 2.50-8.60
≥4yr 1.80-8.40 1.80-8.40
≥7yr 1.50-5.00 1.50-5.00
≥13yr 1.50-4.50 1.50-4.50
≥18yr 1.10-3.40 1.10-3.40
Absolute Monocytes (103/uL)
Age Male Female
>0d 0.00-1.90 0.00-1.90
>1d 0.00-1.70 0.00-1.70
>5d 0.10-1.70 0.10-1.70
>28d 0.21-1.60 0.21-1.60
≥1yr 0.15-1.20 0.15-1.20
≥4yr 0.32-1.10 0.32-1.10
≥12yr 0.27-1.10 0.27-1.10
≥15yr 0.35-1.00 0.35-1.00
≥18yr 0.30-1.10 0.30-1.10
Absolute Eosinophils (103/uL)
Age Male Female
>0d 0.20-0.70 0.20-0.70
≥18yr 0.00-0.50 0.00-0.50
Absolute Basophil (103/uL)
Age Male Female
>0d 0.03-0.11 0.03-0.11
>28d 0.02-0.17 0.02-0.17
≥1yr 0.02-0.12 0.02-0.12
≥4yr 0.00-0.20 0.00-0.20
≥18yr 0.00-0.30 0.00-0.30
Atypical Lymphocytes (%)
Age Male/Female
>0d 0-6
Band Neutrophils  (%)
Age Male/Female
>0d 0-5

 

Reject Due To

 

Hemolysis

Mild:

ok

Gross:

ok

Lipemia

Mild:

ok

Gross:

ok

Icterus

Mild:

ok

Gross:

Reject

 

Day(s) Test Set Up

Monday through Sunday

CPT Coding

85007

Performing Laboratory

Huntsville Hospital Laboratory/Madison Hospital Laboratory