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Test Code CYCISO Cyclospora and Isospora Stain

Methodology

Auraminic and Modified Kinyoun Stains

Specimen Requirements

Submit specimens in O&P collection vials.  Fresh specimens must arrive within 2 hours if stored at Room Temp or 3 days if refrigerated to be acceptable.

Collect specimen as follows:
1. Lift toilet seat, and cover toilet bowl with a large sheet of plastic wrap. Make a depression in plastic wrap. With toilet seat lowered, pass stool specimen directly onto plastic wrap. (Rectal swab is not acceptable.)

2. Alternatively, collect stool specimen in a clean, dry, wide-mouth container. Do not use toilet paper to collect specimen. Do not remove specimen from toilet water.
3. Specimen must be submitted in an O&P collection vial. Follow instructions accompanying the vial(s) for collection procedure.
4. Label container/vial(s) with patient’s information, date and actual time of collection, and type of specimen.

5. Forward specimen promptly.

Note:  1. Maximum storage for fresh specimens is 2 hours at ambient temperature or 3 days if refrigerated. Maximum storage for specimens in an O&P vial at ambient temperature is 2 months or until the expiration date on vial(s), whichever is earlier.

2. Routinely, only 1 stool specimen per day should be collected for O&P testing. After the third hospital day, it is generally recommended that Clostridium difficile toxin testing should be performed on patients with diarrhea, not O&P testing.

3. Specimens will not be processed if transport/storage requirements are exceeded (this will be noted in the report).

4. Contamination of stool specimen with urine or barium is not acceptable.

Reference Values

No Cyclospora oocysts seen

Day(s) Test Set Up

Monday through Friday

CPT Coding

87015 - Concentration

87206 - AF stain

Specimen Stability

For fresh stool specimens:

2 hours if stored at Room Temp

3 days if stored Refrigerated

Performing Laboratory

Huntsville Hospital Laboratory